Composite

Part:BBa_K2497999:Experience

Designed by: Catrin Schuler   Group: iGEM17_Stuttgart   (2017-10-27)


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Azure Keratin Assay: iGEM Team Stuttgart 2017

Azure keratin (Sigma Aldrich: ) was used as substrate.

Keratin_azure_assay_1.png

Extinction of Azo-keratine conversion over time of keratinase KerP. Measurements of enzyme activity was performed at three timepoints (0h, 3h, 24h). Intracellular enzyme activity was measured.

Azure_keratin_assay_2.png

Extinction of Azo-keratine conversion over time of keratinase KerP. Measurements of enzyme activity was performed at three timepoints (0h, 3h, 24h). Extracellular enzyme activity was measured

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